This course aims to ensure alignment of the operating procedure for acaricide resistance tick bioassays testing and enhancing the capacity of laboratory workers to diagnose and monitor acaricide resistance in ticks.
Study time
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2.5 hours.
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Languages currently available
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English.
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Target audience
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Laboratory workers (e.g. technicians, operators) responsible for performing in vitro tick bioassays.
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Learning objectives
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- Identify and select appropriate acaricide resistance test and tick samples for use in in vitro bioassays.
- Describe standard laboratory procedures for handling, incubating, and rearing engorged female ticks, eggs, and larvae.
- Describe the larval packet test (LPT).
- Describe the adult immersion test (AIT).
- Interpret bioassay results.
- Explain quality control measures to ensure the reliability and reproducibility of the bioassay data.
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Course structure
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The course includes five mandatory self-paced modules followed by a multiple-choice assessment.
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Topics covered
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- Principles of in vitro bioassays (LPT and AIT).
- LPT protocols.
- AIT protocols.
- Procedures for collecting, handling, and rearing ticks.
- Analysis of bioassay results and communication of findings.
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